Meet Devon Jones, undergraduate researcher

The second undergraduate to share her experience is Devon Jones, who is one of the newer undergraduates in the lab.  Since January, Devon has been working with with one of the graduate students, Shayen Sreetharan, on a mouse project.  This summer, she is starting to get her feet wet in the fish lab.  Devon will start her senior thesis in the lab this fall.  

Devon Jones, Undergraduate Researcher

Devon JonesEducational Background:

H.BSc. Biology, Physiology Specialization, Level IV, McMaster University

I entered the Wilson lab in January of 2016, halfway through the third year of my undergraduate degree. Having only been a part of the lab for a short time, I feel like I have a lot to learn, but I’m slowly making progress. The majority of the work I have been involved in so far has been assisting with Shayen Sreetharan’s M. Sc. Thesis project. Shayen is studying the effects of varying amounts of low dose gamma irradiation in utero on C57Bl/6 mice. These low doses are similar to the quantity of radiation that would be administered during a brief x-ray or other form of medical imaging test. One of his main points of interest is how these low doses during the fetal period can impact the blood pressure of the pups at about 16 weeks of age. My primary focus for the summer is collecting these blood pressure measurements. I am also doing some data analysis, as well as learning some of the techniques other graduate students use on their projects. In the fall, I will begin working on my senior thesis, which will focus primarily on fludamide exposures and the impacts on androgen receptors and CYP expression.

Still being new to the lab atmosphere, I’m in the process of accumulating crucial skills as well as learning regular maintenance and other important components to helping the entire lab function smoothly as a whole. The biggest thing I’m starting to realize is how little you know about science before you try lab work. Classes and tutorials can only teach students so much about where results come from, and what procedures actually involve. When you read a paper, or listen to a lecture, you have no concept of what is actually involved in producing the end result. I probably learned about how the PCR process worked 3 times during my undergrad, but until trying it in real life, I didn’t really grasp how much work and time are involved in executing the process. Additionally, no paper ever mentions the number of pipette tips that need to be autoclaved, or how long an autoclave cycle takes, in order to obtain your molecular biology results. The big picture is so much bigger than I expected! I think it is important for every undergraduate student to get involved in a lab setting to learn about how these things work. Whether it is running a behavioural study or a pharmaceutical exposure, you can’t learn this stuff in a simulated 3 hour lab slot. I definitely wish I had gotten involved in this lab sooner than I did, but I plan to make the most of my time here!

Meet Abigail Lee, undergraduate researcher

Over the next months, I am asking the undergraduate students in my lab to write about their experiences and research in the Wilson Tox Lab.  We are very lucky that many undergraduates are with us for several years as volunteers, experiential learning course students, thesis students, and summer researchers.  The first undergraduate to share her experience is Abigail Lee, who has done all of the above.  Since her second year of undergraduate, Abby has been a near constant in the lab, so much so that we are having a hard time facing that after this summer, she will leave us and move on to medical school.  During her undergraduate degree, Abby has developed into a competent, skilled, and knowledgeable researcher.  She is will be greatly missed.

Abigail Lee, Undergraduate Researcher 

Educational Background:

  • H.BSc. Biology, Minor Biochemistry, McMaster University, 2016

I am currently working on a project studying the effects of pharmaceuticals on the budding rate, morphology, feeding behavior and regenerative capacity of brown Hydra.

I’ve been a part of the Wilson lab since the second year of my undergraduate career and needless to say this has become like my second home! I was lucky enough to be taken in as a volunteer, where I began familiarizing myself with the lab environment by being the primary animal husbandry person for our zebrafish. Soon, I began splitting my time working on the Lake Whitefish species, where I was a given a project to determine the effects short term heat shocks on the development of Lake Whitefish. I was awarded an NSERC USRA in the summer of my second year where I wrote my first paper about the research I completed with Lake Whitefish (Read my paper here, shameless plug: doi: 10.1016/j.jtherbio.2016.01.010.). In my third year, I switched gears towards a molecular biology approach and completed an independent research project (Science 3RP3) studying the effects of different temperature regimes on gene expression of oxidative phosphorylative genes in Lake Whitefish. Finally, for my senior thesis, I studied the effects of fluoxetine and carbamazepine on multiple different endpoints in Brown Hydra. I am currently working on writing a manuscript to publish this work. In my final summer work term, along with completing my Hydra project, I am also helping with Shayen Sreethanran’s ( M.Sc. thesis studying the effects of low dose radiation in mice.

I would not be the person I am today without this entire experience in the Wilson Lab. I’ve been a volunteer, an independent research course student, a thesis student and a summer work student and I can walk away confidently saying that I have learned so many different techniques and skills that will serve me for a lifetime.

Some important things that I learned while working in the Wilson lab are:

1) Always try something new; don’t only cling to what you know but immerse yourself into something new, without fear, because you never know what you may learn.

2) Never give up; research does not always work out your way. Those ‘not statistically significant’ results are still results! Keep working hard!

3) Don’t underestimate any experience; I began with racking tips and cleaning poop out of fish tanks, but look where that brought me! Every experience will take you closer to where you want to be.

The people in the lab have become like my family; they took me under their wings and have shaped me to become the scientist I am today. The collaborative atmosphere allowed me to learn from the best and the satisfaction of working my way up from a volunteer to an independent researcher is incomparable to any other experience. It’s true that hard work and determination pay off!

My time at McMaster University is nearing an end and I will be attending the University of Toronto to pursue an M.D. But this won’t be the last time the research world hears my name, I hope to eventually pursue a Ph.D to combine my love of research with my passion for medicine.

So, you want to be a session chair?

Michael Lim is a MSc student in the Wilson Tox Lab and recently had his first experience as a conference session chair.  He shares his experiences in this post…

Why hello there! If you’re reading this, chances are that you’re likely in the same position I was in a few months ago; interested in an opening to be a session chair for a conference, but not quite sure what the job entails. Well rest assured, as I’ll share my recent experience being a chair at the 55th Canadian Society of Zoologists (CSZ) Annual Meeting based at Western University.

As a second year McMaster University Master’s student (try saying that 10 times fast!) in the Wilson Toxicology Lab, I’ve been fortunate enough to attend several conferences and meetings. In fact, I attended the 54th CSZ Meeting last year at the University of Calgary. If you’re a biologist, and especially if you’re Canadian, chances are that you’ve at least heard of the CSZ meeting, if you haven’t attended it in person. For those of you who haven’t yet heard about CSZ, their annual meeting serves as an opportunity for biologists from across the country and beyond to gather and share their research, celebrating ground-breaking studies and fantastic presentations across both platform and poster presentations. There is an incredibly wide variety of work, ranging from tiny organisms like daphnia all the way to multi-level African ecosystem dynamics.

As hundreds of members come to share their research every year, presentations end up being run in several consecutive sections throughout the day for the entire conference. Each platform presentation section is further split into different concurrent sessions which include research on a specific topic such as hypoxia, environmental contaminants, and molecular physiology. In order for each session to run smoothly and remain on schedule, CSZ members are asked to volunteer themselves as session chairs. This brings us to the heart of this post; what is a session chair, and what do they do? While I can’t speak for every session chair (there were over 20!), I can share some of my personal experiences and give some tips both on presenting and being a chair.

As I briefly mentioned before, this was my second time at a CSZ meeting, and this time around I decided I wanted to better understand how the conference was run and develop my presentation skills in a role that required more responsibility and flexible thinking. When the chance to be a session chair was offered, I knew that this was the perfect opportunity. That is not to say I had no doubts or questions. As a self-proclaimed introvert, like many scientists are, the idea of presiding over other researchers (some of whom are leaders in their fields) filled me with a sense of nervousness. What if I completely botch their name, or the name of the species they worked on? What if there are no questions and I subject the audience to intense awkward silence? What if I act like a literal “chair”, freezing up in front of the crowd? It is in moments of nervousness like these that I remember some advice I got from one of my teachers in middle school, which I’ll now pass on to you.

First, take a moment to step back and detach yourself. Becoming too focused and worrying about every tiny detail won’t help you. Prepare for things that are manageable, such as asking each presenter how they would like to be addressed and clarifying any pronunciation questions. In order to keep everything on schedule, I recommend setting up signals ahead of time with each presenter, preferably at least two signals spaced several minutes apart so that the presenter will have time to wrap up concisely. Some common signals to use include raising a piece of paper, or standing up at the side of the stage, but still in view of the presenter.

For everything else, you just need to accept that problems will always happen, and that you’ll just need to calmly address them one at a time. For example, during my session, there ended up being a scheduling conflict, which resulted in needing to move the speaking order around.

Second, being nervous is perfectly normal. Use it as a tool when preparing for your presentation, to focus on being as clear and direct as possible. During the presentation itself, turn the nervousness into energy to help your presentation stand out. This can come in many forms such as projecting your voice more strongly, using dynamic hand gestures, or even becoming spontaneously witty. This will not happen right away. It may even take tens to hundreds of presentations; but the important thing is to keep on practicing. If you don’t try to change, you never will.

Finally, and possibly most importantly, you must realize that your audience isn’t trying to tear you down. Without a doubt, you’ll come across a handful of people who enjoy making others feel awkward or uncertain, but the vast majority of your audience is genuinely interested in what you’re working on, and they want to see you develop and succeed as a researcher.

In closing, being a chair is something I definitely recommend everyone should try at least once in their graduate career. I know that it can sometimes be hard to know if your preparation and perseverance pay off, but in the words of someone who thanked me for running a good session, “As long as you keep everything moving and add your own fun flair, that’s all anyone can ask for.”

Did you know my students blog?

Shayen Sreetharan is a new MSc student in the WilsonToxLab this year but he isn’t really new to us.  Shayen started working in the lab during the 3rd year of his undergraduate degree and we have convinced him to stay.  His first project involved describing Lake Whitefish development, a project that has lead to a publication in the Journal of Fish Biology.  Now undergrads that publish are not rare in my lab but undergrads with first authorship on a published paper prior to graduation?  That is very special.  Shayen did his senior thesis in my lab in 2014-2015, working on a multiple stressor effects in the rainbow trout cell line, RTG-2.  Now he has started graduate school but doing it in his own way.  Shayen’s project is a bit of a stretch for us fishy folks because he is working on mice in a  collaboration between the WilsonToxLab and Dr. Doug Boreham’s lab at NOSM.  Below is a post from Shayen, describing his new science blog:

Dose, Dose Rate & Depolarization: A Blog of Science

I started the blog: Dose, Dose Rate & Depolarization on my website as a means of sharing interesting papers, news or just random thoughts on the two (albeit unrelated) areas of radiation biology and paediatric cardiology. I’ve always been interested in blogging and seeing the appeal of blog posts by Dr. Joanna Wilson here on the WilsonToxLab website helped make the decision to create one of my own!

Being co-supervised by Dr. Douglas Boreham, Director of Medical Sciences at the Northern Ontario School of Medicine (NOSM) for the past few years, I have had a growing interest in radiation biology. Previously, my academic background prior to joining the lab was focussed on physiology (including whole animal/human anatomy and physiology, cell physiology, pathophysiology and physiological adaptations to stressful/novel environments). As I learned more through reading papers for my undergraduate thesis and just working alongside Doug’s graduate students, I gained an appreciation for the intricacies of radiation biology. What was truly of interest to me was not only the biological effects of ionizing radiation exposure but the area of low dose radiation biology. The harmful effects of high doses of radiation are well known, however there is active discussion regarding the effects at the low dose and low dose rate region.

The other half of the blog features a rather different area: paediatric cardiology. Working for Dr. Robert Hamilton , a cardiologist (electrophysiology) at the Hospital for Sick Children in Toronto should make my interest in the field self-explanatory. My supervisor focuses on various rhythm disorders in children including a number of diseases that I’ve been a part of: ARVC/D, Brugada Syndrome  and Long Q-T Syndrome. I have been fortunate to be involved in both wet-lab (working on CRISPR/Cas9 of a cardiomyocyte cell line) and clinical research experience allowing me to be involved in ameliorating patient care from the lab bench.

Feel free to check out the blog and leave any thoughts or questions in the comment section!

First Conference Experience – Kristine Tells All

From Kristine Hammill, a MSc Candidate in the WilsonToxLab, about her first conference experience at SETAC 2015!

DSCF4006As a Master’s student in the Wilson Lab I’ve been experiencing many of my graduate school firsts. Most recently, this month I attended my first large-scale conference, the Society of Environmental Toxicology and Chemistry’s North America 36th Annual Meeting, otherwise known as SETAC Salt Lake City.

Having completed my undergraduate degree in environmental science, SETAC was an acronym I heard a lot, but yet knew very little about. Needless to say, I was stoked to have the opportunity to attend my first SETAC, but extremely nervous for my first significant platform presentation that awaited me in Salt Lake City.

My nerves were quickly calmed upon my arrival as I received a warm welcome into the SETAC family. Throughout the course of the week, I couldn’t help but think of the event as a “Toxicologist’s Christmas” or a “Contiki Tour for Environmental Scientists”. By day you were nerding out with some great environmental tox talks, and by night you were rocking out on the dance floor with your new found friends and the scientists you admire. There was even time in there to explore the city.

I greatly appreciated the opportunity to hear about what else was going on in the world of environmental toxicology outside of my own little niche. Once joining the ranks of grad school it’s easy to get lost in the depth of your project and lose touch with the breadth of knowledge that comes naturally in undergraduate studies. It also provided a peace of mind to my inner environmental scientist: it doesn’t matter if I’m not tackling all the environmental issues, rest assure, there are many individuals out there working on their piece of the puzzle to contribute to the bigger picture.

As for my presentation, it turns out I had nothing to worry about. Before I presented, I was told it was a very forgiving crowd at SETAC, and my experience as both a presenter and audience member reflected that. Above all, I was happy to see that no one was being egotistical with the question period. Intelligent questions were asked with poise, and many questions were backed with their own explanation so it was understood where the asker was coming from.

All in all, it was a wonderful first experience at SETAC. I’m looking to head out into the working world after my Master’s and can’t say when I’ll be back again, but I hope that I’m lucky enough to find an employeer that will support my attendance.

With that, I’ll leave you with my top 5 tips for first-time SETAC attendees:

1. Participate in the buddy program.  My seasoned SETACer showed me the ropes, gave me great tips for the conference, provided insight into the consulting industry and government sector, and was able to introduce me to her network. Added bonus – she had a second first time attendee buddy who was also the only person from her lab attending, so we were able to navigate the waters together.

2. Sign-up for structured activities.  Not only do you benefit from the actual activities, which was my own initial draw, but it’s a great way to meet people. It’s a lot easier to start a conversation with someone when you’re sitting at the same lunch table then when you’re walking into a warehouse sized room with hundreds or thousands of people.

3. Take advantage of the technical content.  It’s great to hear about what else is going on in your particular area of focus, and to pick-up some tips and tricks for your own project, but SETAC is also a chance to explore the other areas of tox that you’ve always been interested in but may not have time for. Some sessions are even recorded and posted online so you can listen to what you missed at your own leisure.

4. Attend meetings/advisory groups.  SETAC is an inclusive group and you don’t need to receive a personal invitation to check out advisory groups or attend meetings (albeit those that are for the executive will be flagged). If you work with pharmaceuticals, go check out the Pharmaceutical Advisory Group. If you work with metals, go check out the Metals Advisory Group. It’s a great way to see how academia, government and industry work together and how research in the field is being shaped. Also be sure to check out the North America Student Advisory Council (NASAC) Open Assembly and hear about what the students of SETAC have going on.

5. Explore the vendors…ALL of them…As a Master’s student looking to graduate in less than a year’s time, I planned to hit up the vendors to network and ask those burning questions to potential employers. I set out to tackle the consulting companies and hadn’t considered exploring the publishers. I’m not getting a PhD – what use would I be to them? Well, after being sent to Wiley’s booth during the Career Navigation event, I learned that there are opportunities for MSc grads with a passion for science communication that sound right up my alley.